MAPPIT: a versatile tool to study cytokine receptor signalling. (19th November 2008)

• Record Type: Journal Article
• Authors: Lemmens, Irma; Lievens, Sam; Tavernier, Jan
• Is Part Of: Biochemical Society transactions. Volume 36:Number 6(2008)
• Page Start: 1448
• Page End: 1451
• Publication Date: 2008-11-19
• Subjects: cytokine receptor -- mammalian protein–protein interaction trap (MAPPIT) -- signal transducer and activator of transcription (STAT) -- signal transduction -- suppressor of cytokine signalling (SOCS) -- Toll-like receptor (TLR); Biochemistry -- Congresses; 572
• DOI: 10.1042/BST0361448
• Languages: English
• ISSNs: 0300-5127

Abstract

Abstract : MAPPIT (mammalian protein–protein interaction trap) is a cytokine receptor-based two-hybrid method that operates in intact mammalian cells. A bait is fused C-terminally to a STAT (signal transducer and activator of transcription) recruitment-deficient receptor, whereas the prey is linked to functional STAT-binding sites. When bait and prey interact a ligand-dependent complementation of the STAT recruitment deficiency occurs, leading to activation of a STAT-responsive reporter. MAPPIT is very well suited to study protein interactions involving activated cytokine receptors as the technique allows modification of the bait protein in a physiologically optimal environment.