Selective retention of organic phosphate esters and phosphonates on aluminium oxide. Issue 5 (May 1986)

• Record Type: Journal Article
• Authors: Coletti-Previero, M-A.; Pugnière, M.; Mattras, H.; Nicolas, J. C.; Previero, A.
• Is Part Of: Bioscience reports. Volume 6:Issue 5(1986)
• Page Start: 477
• Page End: 483
• Publication Date: 1986-05
• Subjects: phosphate esters -- phosphonate esters -- aluminium oxide -- purification by aluminium oxide; Molecular biology -- Periodicals; Cytology -- Periodicals; 572.8
• DOI: 10.1007/BF01116139
• Languages: English
• ISSNs: 0144-8463

Abstract

Abstract : Compounds containing the −PO3 H2 function, such as monoesters of phosphoric acid and phosphonic acids, specifically bind to aluminium oxide in aqueous solution under experimental conditions where non-phosphorylated compounds are completely desorbed. The bound organic phosphate can be specifically displaced by aqueous solution of inorganic phosphates thus allowing their separation or detection by a technique similar to that of affinity chromatography. The consequences of this finding for phosphate compound biochemistry are discussed.