The Histidine Decarboxylase (HDC) Gene of Tetrahymena Pyriformis is Similar to the Mammalian One. A Study of HDC Expression. Issue 2 (April 1999)

• Record Type: Journal Article
• Authors: Hegyesi, Hargita; Szalai, Cs.; Falus, A.; Csaba, G.
• Is Part Of: Bioscience reports. Volume 19:Issue 2(1999)
• Page Start: 73
• Page End: 79
• Publication Date: 1999-04
• Subjects: Histidine decarboxylase -- Tetrahymena -- gene sequencing -- evolution -- histamine; Molecular biology -- Periodicals; Cytology -- Periodicals; 572.8
• DOI: 10.1023/A:1020102308791
• Languages: English
• ISSNs: 0144-8463

Abstract

Abstract : RNA was isolated from Tetrahymena pyriformis GL and using human histidine decarboxylase (HDC) gene primers, the RT-PCR product was sequenced. A fraction containing 207 base pairs was compared to the published sequences of prokaryotic and mammalian (rat, mouse and human) HDC cDNA (exons). The HDC-cDNA fraction of Tetrahymena was similar to the mammalian cDNA-s and it was completely different from the prokaryotic HDC-gene. The results indicate the presence of a mammalian-like HDC-gene already in a unicellular eukaryote organism and demonstrates also that the divergence of the prokaryotic–eukaryotic common gene took place already at this low evolutionary level.