High-level transcription of large gene regions: a novel T7 RNA-polymerase-based system for expression of functional hydrogenases in the phototrophic bacterium Rhodobacter capsulatus. (1st February 2005)

• Record Type: Journal Article
• Authors: Drepper, T.; Arvani, S.; Rosenau, F.; Wilhelm, S.; Jaeger, K.-E.
• Is Part Of: Biochemical Society transactions. Volume 33:Number 1(2005)
• Page Start: 56
• Page End: 58
• Publication Date: 2005-02-01
• Subjects: heterologous expression -- membrane protein -- redox enzyme -- Rhodobacter capsulatus -- T7 RNA-polymerase -- uptake hydrogenase; Biochemistry -- Congresses; 572
• DOI: 10.1042/BST0330056
• Languages: English
• ISSNs: 0300-5127

Abstract

Abstract : High-level synthesis of complex enzymes like bacterial [NiFe] hydrogenases, in general, requires an expression system that allows concerted expression of a large number of genes. So far, it has not been possible to overproduce a hydrogenase in a stable and active form by using a customary expression system. Therefore we started to establish a new, T7 -based expression system in the phototrophic bacterium Rhodobacter capsulatus . The beneficial properties of this bacterial host in combination with the unique capacity of T7 RNA polymerase to synthesize long transcripts will allow the high-level synthesis and assembly of active hydrogenase as well as other complex enzymes in the near future.