Endoplasmic reticulum generates calcium signalling microdomains around the nucleus and spindle in syncytial Drosophila embryos. (22nd May 2006)

• Record Type: Journal Article
• Authors: Parry, H.; McDougall, A.; Whitaker, M.
• Is Part Of: Biochemical Society transactions. Volume 34:Number 3(2006)
• Page Start: 385
• Page End: 388
• Publication Date: 2006-05-22
• Subjects: calcium signalling microdomain -- cortex -- Drosophila embryo -- endoplasmic reticulum -- extraembryonic calcium -- mitosis; Biochemistry -- Congresses; 572
• DOI: 10.1042/BST0340385
• Languages: English
• ISSNs: 0300-5127

Abstract

Abstract : Cell cycle calcium signals are generated by inositol trisphosphate-mediated release of calcium from internal stores [Ciapa, Pesando, Wilding and Whitaker (1994) Nature (London) 368, 875–878; Groigno and Whitaker (1998) Cell 92, 193–204]. The major internal calcium store is the ER (endoplasmic reticulum): the spatial organization of the ER during mitosis is important in defining a microdomain around the nucleus and mitotic spindle in early Drosophila embryos [Parry, McDougall and Whitaker (2005) J. Cell Biol. 171, 47–59]. Nuclear divisions in syncytial Drosophila embryos are accompanied by both cortical and nuclear localized calcium transients. Mitosis is prevented by the Ins P 3 antagonists Xestospongin C and heparin. Nuclear-localized transients and cortical transients rely on extraembryonic calcium, suggesting that ER calcium levels are maintained by calcium influx.