Isolation and characterization of a Pseudomonas poae JSU-Y1 with patulin degradation ability and biocontrol potential against Penicillium expansum. (May 2021)

• Record Type: Journal Article
• Authors: Ren, Yun; Yao, Man; Chang, Peipei; Sun, Yemei; Li, Rui; Meng, Di; Xia, Xiaoshuang; Wang, Yun
• Is Part Of: Toxicon. Volume 195(2021)
• Page Start: 1
• Page End: 6
• Publication Date: 2021-05
• Subjects: Penicillium expansum -- Patulin -- Biological degradation -- Pseudomonas poae; Toxins -- Periodicals; Venom -- Periodicals; 615.9
• DOI: 10.1016/j.toxicon.2021.02.014
• Languages: English
• ISSNs: 0041-0101

Abstract

Abstract: Patulin, one of the most common mycotoxins produced primarily by the Penicillium, Aspergillus and Byssochlamys species, is often associated with fruits and fruit-based products. Biodegradation by microbes is an effective method to remove or detoxify mycotoxins. In this study, a bacterial strain with patulin degradation capability was selectively isolated using oxindole, an analogue to patulin, as the sole carbon source, and identified as Pseudomonas poae JSU-Y1 by phylogenetic analysis on the basis of 16S rRNA sequence. This isolated bacterium could inhibit the growth of Penicillium expansum both on plate medium and apple fruit with inhibition ratio of 30.3% and 44.9%, respectively. Up to 87.7% of the initial patulin (2.5 μg/mL) was removed after incubation with Pseudomonas poae JSU-Y1 in liquid medium at 30 °C for 72 h. When challenged with apple juice, 79% of patulin could be degraded by this isolated strain. Additionally, ascladiol was tentatively identified as the patulin degradation intermediate by LC-MS analysis. Taken together, the experiment results indicated that the isolated Pseudomonas poae JSU-Y1 would be a promising bacterial resource to control patulin contamination and toxigenic fungal growth in agricultural products. Graphical abstract: Image 1 Highlights: Pseudomonas poae JSU-Y1 can degrade patulin. P. poae JSU-Y1 exhibits growth inhibition on Penicillium expansum. P. poae JSU-Y1 reduces blue mold rot of apple fruits. Ascladiol is tentatively to be the patulin degradation intermediate.