Specificity of a ctyochemical bioassay for arginine-vasopressin and its validation for plasma measurement. Issue 2 (February 1984)

• Record Type: Journal Article
• Authors: Smith, A.; McIntosh, N.
• Is Part Of: Bioscience reports. Volume 4:Issue 2(1984)
• Page Start: 109
• Page End: 114
• Publication Date: 1984-02
• Subjects: Molecular biology -- Periodicals; Cytology -- Periodicals; 572.8
• DOI: 10.1007/BF01120306
• Languages: English
• ISSNs: 0144-8463

Abstract

Abstract : The total Na+/K + ATP-ase activity of the thick ascending limb of the loop of Henlé may be stimulated by arginine-vasopressin (AVP). Lysine-vasopressin (LVP), oxytocin (OT), and arginine-vasotocin (AVT) produce less than 5% of the enzyme activity induced by the same concentration of AVP. Physiological concentrations of a mixture of other hormones with known activity on the kidney (T3, T4 aldosterone, angiotensin II, and OT) did not significantly increase total Na+/K + ATP-ase activity. Specific AVP antiserum consistently removed greater than 90% of the stimulatory effect of plasma. The concentration of AVP in plasmas from dehydrated subjects was greater than l0 times that of the same subjects hydrated. Intra-assay coefficient of variation was 35% and 52% from 200 μl and 20 μl of plasma respectively. The interassay coefficient of variation was 53% and 55% from plasma pools with high and low AVP content.