The effect of tetracyclines on quantitative measures of osteoclast morphology. Issue 3 (June 1993)

• Record Type: Journal Article
• Authors: Zaidi, Mone; Moonga, Baljit S.; Huang, Christopher L.-H.; Alam, A.S.M. Towhidul; Shankar, Vijai S.; Pazianas, Michael; Eastwood, John B.; Datta, Harish K.; Rifkin, Barry R.
• Is Part Of: Bioscience reports. Volume 13:Issue 3(1993)
• Page Start: 175
• Page End: 182
• Publication Date: 1993-06
• Subjects: tetracyclines -- 4-dedimethylaminotetracycline -- osteoclastic resorption -- osteoclast Ca2+ receptor; Molecular biology -- Periodicals; Cytology -- Periodicals; 572.8
• DOI: 10.1007/BF01149962
• Languages: English
• ISSNs: 0144-8463

Abstract

Abstract : We report the effects of the tetracycline analogues 4-dedimethylaminotetracycline (CMT-1) and minocycline on osteoclast spreading and motility. Both agents influenced the morphometric descriptor of cell spread area, ρ, producting cellular retraction or an R effect (half-times: 30 and 44 minutes for CMT-1 and minocycline, respectively). At the concentrations employed, the tetracycline-induced R effects were significantly slower than, but were qualitatively similar to, those resulting from Ca 2+ "receptor" activation through the application of 15 mM-[Ca 2+ ] (slopes: −1.25, −0.18, and −4.40/minute for 10 mg/l-[CMT-1], 10 mg/l-[minocycline] and 15 mM-[Ca 2+ ], respectively). In contrast, the same tetracycline concentrations did not influence osteoclast margin ruffling activity as described by μ, a motility descriptor known to be influenced by elevations of cellular cyclic AMP. Thus, the tetracyclines exert morphometric effects comparable to changes selectively activated by occupancy of the osteoclast Ca 2+ "receptor" which may act through an increase in cytosolic [Ca 2+ ].