Significant Changes in the Levels of Secreted Cytokines in Brains of Experimental Antiphospholipid Syndrome Mice. (22nd February 2012)

• Record Type: Journal Article
• Authors: Menachem, Assaf; Chapman, Joab; Katzav, Aviva
• Other Names: de Carvalho Jozélio Freire Academic Editor.
• Is Part Of: Autoimmune diseases. Volume 2012(2012)
• Publication Date: 2012-02-22
• Subjects: Autoimmune diseases -- Periodicals; 571.97305
• DOI: 10.1155/2012/404815
• Languages: English
• ISSNs: 2090-0422

Abstract

Abstract : Antiphospholipid syndrome (APS) is characterized by thromboses and neuropsychiatric manifestations possibly linked to brain inflammation. In order to examine the levels of proinflammatory and anti-inflammatory cytokines in experimental APS (eAPS) mice brains, we measured the levels of TNF- α, IFN- γ, and IL-10 in brain homogenates (cytosolic fractions) and in brain slices (secreted level) at 6, 15, and 24 weeks after immunization. We induced eAPS by immunization of Balb/c mice with β 2 -glycoprotein I ( β 2 GPI), the major autoantigen in the disease and controls with adjuvant alone. We found increased levels of secreted TNF- α in eAPS mice for the entire experiment period. Cytosolic and secreted IL-10 and IFN- γ levels in eAPS mice were lower at 6 and 15 weeks and higher at 24 weeks after immunization. The results suggest that brain disease in APS is associated with significant and complex changes in proinflammatory and anti-inflammatory cytokines.