Two stages of the differentiation of HL-60 cells induced by 1α, 25 dihydroxyvitamin D3; Commitment by 1α, 25 dihydroxyvitamin D3 and promotion by DMSO. Issue 1 (January 1986)

• Record Type: Journal Article
• Authors: Momoi, Takashi; Senoo, Haruki; Yoshikura, Hiroshi
• Is Part Of: Bioscience reports. Volume 6:Issue 1(1986)
• Page Start: 95
• Page End: 101
• Publication Date: 1986-01
• Subjects: differentiation -- Hl-60 cells -- 1α, 25 dihydroxyvitamin D3 -- commitment -- promotion -- DMSO; Molecular biology -- Periodicals; Cytology -- Periodicals; 572.8
• DOI: 10.1007/BF01145184
• Languages: English
• ISSNs: 0144-8463

Abstract

Abstract : The differentiation of HL-60 cells induced by 1α, 25 dihydroxyvitamin D3 was found to be separated into two stages, i.e. commitment and promotion. Most of the HL-60 cells were committed to monocyte/macrophage lineage by pretreatment with 1α, 25 dihydroxyvitamin D3 (5–50 ng/ml) for 18–24 hr. The promotion in the second stage was inducer and lineage independent; treatment with 1.25% DMSO for 2 or 3 days promoted the differentiation of the committed HL-60 cells by 1α, 25 dihydroxyvitamin D3 into monocyte/macrophage lineage, but not granulocyte lineage.