Nanozymes "Artificial Peroxidase": Enzyme Oxidase Mixtures for Single‐Step Fabrication of Advanced Electrochemical Biosensors. Issue 6 (17th March 2021)

• Record Type: Journal Article
• Authors: Komkova, Maria A.; Andreeva, Ksenia D.; Zarochintsev, Alexander A.; Karyakin, Arkady A.
• Is Part Of: ChemElectroChem. Volume 8:Issue 6(2021)
• Page Start: 1117
• Page End: 1122
• Publication Date: 2021-03-17
• Subjects: nanozymes -- electrocatalysis -- artificial peroxidase -- oxidase -- electrochemical biosensors -- Prussian Blue; Electrochemistry -- Periodicals; 541.37
• DOI: 10.1002/celc.202100275
• Languages: English
• ISSNs: 2196-0216

Abstract

Abstract: We report the single‐step fabrication of advanced electrochemical biosensors upon drop‐casting of "artificial peroxidase", that is, oxidase‐containing mixtures. Compared to bienzyme biosensors, we substitute peroxidase with more active and stable nanozymes based on catalytically synthesized Prussian Blue. The electroactivity of nanozymes in the sensing membrane was facilitated with carbon black nanoparticles, which resulted in record sensitivity of the H2 O2 transducer exceeding 1.1 A M −1 ⋅ cm −2 . Both glucose and lactate oxidases were co‐immobilized with nanozymes in alkoxysilane or perfluorosulfonated ionomer membranes. The proposed drop‐casting approach results in biosensors that are advantageous over those conventionally produced upon layer‐by‐layer immobilization in terms of i) almost an order of magnitude increased sensitivity, ii) unsurpassed biosensor‐to‐transducer sensitivity ratio reaching the value of 0.3, and iii) three times extended operational lifetime. Abstract : Artificial intelligence : Single‐step fabrication of advanced electrochemical biosensors is achieved by drop‐casting. Record sensitivity of the hydrogen peroxide transducer based on "artificial peroxidase" is demonstrated. An unsurpassed biosensor‐to‐transducer sensitivity ratio, reaching the value of 0.3, is reported. In addition, an order of magnitude increase in sensitivity and three times extended operational lifetime of the biosensor is shown.