Induction of metallothionein and methallothionein mRNA in rainbow-trout liver following cadmium treatment. Issue 8 (August 1984)

• Record Type: Journal Article
• Authors: Bonham, Keith; Gedamu, Lashitew
• Is Part Of: Bioscience reports. Volume 4:Issue 8(1984)
• Page Start: 633
• Page End: 642
• Publication Date: 1984-08
• Subjects: Molecular biology -- Periodicals; Cytology -- Periodicals; 572.8
• DOI: 10.1007/BF01121016
• Languages: English
• ISSNs: 0144-8463

Abstract

Abstract : A low-molecular-weight cadmium-binding protein was induced in the livers of rainbow trout ( Salmo gairdnerii ) following a series of intraperitoneal injections of cadmium chloride. The subsequent purification and amino acid analysis of this protein showed it to be a true metallothionein. As in higher organisms, two major forms of metallothionein appeared to be present in the liver following cadmium treatment. Following a similar induction procedure total RNA was also isolated and shown to contain high levels of metallothionein-mRNA activity when assayed in a wheat-germ cell-free translation system. This activity was present in the Poly-A + -containing fraction of the total RNA. The bulk of this mRNA activity was shown to be in the 8–10S region of a sucrose gradient.