Effects of mitogenic agents upon glucocorticoid action in human tonsillar T-lymphocytes. Issue 1 (February 1990)

• Record Type: Journal Article
• Authors: Altiok, S.; Bermek, E.
• Is Part Of: Bioscience reports. Volume 10:Issue 1(1990)
• Page Start: 69
• Page End: 72
• Publication Date: 1990-02
• Subjects: glucocorticoid -- mitogenesis -- receptor -- T-lymphocyte; Molecular biology -- Periodicals; Cytology -- Periodicals; 572.8
• DOI: 10.1007/BF01116853
• Languages: English
• ISSNs: 0144-8463

Abstract

Abstract : The treatment of human tonsillar T-lymphocytes with 4-phorbol 12-myristate 13-acetate (PMA), resulted in about two fold increase in glucocorticoid receptor (GR) number, without any significant change in the receptor affinity. This increase disappeared in the presence of cycloheximide. Alone, PMA and calcium inophore A23187 did not affect, but together stimulated, like phytohaemagglutinin (PHA), leucine and, in particular, thymidine incorporation. PMA enhanced slightly the stimulatory effect of PHA. Alone, these agents failed to alter the suppressive effect of dexamethasone on thymidine and leucine incorporation; however, PMA-A23187 and PMA-PHA combinations appeared to antagonize the supression by dexamethasone.