O-glycan regulation of apoptosis and proliferation in colorectal cancer cell lines. (25th October 2007)

• Record Type: Journal Article
• Authors: Patsos, G.; Robbe-Masselot, C.; Klein, A.; Hebbe-Viton, V.; Martin, R. San; Masselot, D.; Graessmann, M.; Paraskeva, C.; Gallagher, T.; Corfield, A.
• Is Part Of: Biochemical Society transactions. Volume 35:Number 5(2007)
• Page Start: 1372
• Page End: 1374
• Publication Date: 2007-10-25
• Subjects: apoptosis -- benzyl-O-N-acetyl-D-galactosamine -- colorectal cancer -- O-glycosylation -- mucin -- signal pathway; Biochemistry -- Congresses; 572
• DOI: 10.1042/BST0351372
• Languages: English
• ISSNs: 0300-5127

Abstract

Abstract : Cell growth pathways are mediated through protein–glycan interactions including O-glycosylation. Investigation of these growth pathways can be carried out using appropriate inhibitors to identify stage-specific events. We have adopted this approach to study a group of benzyl- O - N -acetyl-D -galactosamine analogues in human colorectal cancer cell lines. Exposure to O-glycan inhibitors resulted in the induction of apoptosis, a block in proliferation, accumulation of intracellular aryl-glycans and changes in related genes as detected by gene array. Colorectal cancer cell lines susceptible to the inhibitors showed growth arrest with all compounds. However, a differential action of each inhibitor was detected in the pattern of genes affected and in the structure of aryl-glycans formed.