A radioimmunocytological quantitative method for the rapid detection of ras oncogene p21 protein in mammalian cells. Issue 4 (April 1986)

• Record Type: Journal Article
• Authors: Spandidos, Demetrios A.; Dimitrov, Todor; Kam, Ephraim Y.
• Is Part Of: Bioscience reports. Volume 6:Issue 4(1986)
• Page Start: 375
• Page End: 379
• Publication Date: 1986-04
• Subjects: Radioimmuno assay -- ras oncogene -- oncogene -- p21 protein; Molecular biology -- Periodicals; Cytology -- Periodicals; 572.8
• DOI: 10.1007/BF01116424
• Languages: English
• ISSNs: 0144-8463

Abstract

Abstract : In order to provide a sensitive and quantitative detection method of ras p21 at the cytological level, the monoclonal antibody Y 13 259 and iodinated protein A were used to locate the ras protein in various mammalian cell lines. The subsequent autoradiograph can be analysed by a computer-assisted system which showed in these reported experiments that the relative levels of p21 detected in these cells corresponded to results obtained earlier using conventional biochemical methods.