Interaction of alpha-sarcin and gelonin with Cibacron blue. Issue 10 (October 1986)

• Record Type: Journal Article
• Authors: Sperti, Simonetta; Montanaro, Lucio; Rambelli, Fioretta; Zamboni, Mariacristina
• Is Part Of: Bioscience reports. Volume 6:Issue 10(1986)
• Page Start: 901
• Page End: 908
• Publication Date: 1986-10
• Subjects: alpha-sarcin -- gelonin -- Cibacron blue -- blue dextran -- polynucleotides -- poly(U)-Sepharose -- ribosome-inactivating proteins; Molecular biology -- Periodicals; Cytology -- Periodicals; 572.8
• DOI: 10.1007/BF01116244
• Languages: English
• ISSNs: 0144-8463

Abstract

Abstract : Alpha-sarcin and gelonin, two proteins which inactivate the 60S ribosomal subunit, interact with Cibacron blue and bind to blue dextran-Sepharose, from which they are partially desorbed by nucleoside triphosphates and, more efficiently, by homopolynucleotides. It is further shown that the two proteins bind to poly(U)-Sepharose and that homopolynucleotides protect dilute solutions of both inhibitors from inactivation. It is suggested that the presence of a polynucleotide site on alphasarcin is related to its ribonuclease activity. The existence of a similar site on gelonin might be a clue to its yet unknown mechanism of action.