Characterization of a membrane-specific tubulin isoform by peptide mapping. Issue 10 (October 1986)

• Record Type: Journal Article
• Authors: Hargreaves, Alan J.; Avila, Jesus
• Is Part Of: Bioscience reports. Volume 6:Issue 10(1986)
• Page Start: 913
• Page End: 919
• Publication Date: 1986-10
• Subjects: synaptic plasma membranes -- tubulin isoform -- peptide mapping; Molecular biology -- Periodicals; Cytology -- Periodicals; 572.8
• DOI: 10.1007/BF01116246
• Languages: English
• ISSNs: 0144-8463

Abstract

Abstract : A membrane-specific tubulin-like protein, found in preparations of synaptic plasma membranes and brain mitochondria, was analyzed by chemical and proteolytic peptide mapping to determine which part of the molecule was different from cytoplasmic tubulin. The membrane polypeptide was identical to alpha tubulin in the first two-thirds of the molecule containing the amino terminal, as found by peptide mapping. However, some differences were observed in the peptide maps of the carboxy terminal one third of the molecule which includes a domain that is important in the regulation of tubulin self-assembly.