Small molecule-induced trinucleotide repeat contractions during in vitro DNA synthesis. Issue 26 (1st March 2021)

• Record Type: Journal Article
• Authors: Dohno, Chikara; Hagihara, Masaki; Binti Mohd Zaifuddin, Nursakinah; Nihei, Mizuki; Saito, Kaoru; Nakatani, Kazuhiko
• Is Part Of: Chemical communications. Volume 57:Issue 26(2021)
• Page Start: 3235
• Page End: 3238
• Publication Date: 2021-03-01
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/d1cc00349f
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : A CAG repeat binding molecule, NA, induced efficient repeat contractions during DNA polymerase-mediated primer extension through a CAG repeat template. Abstract : We demonstrated that a synthetic ligand NA, which selectively binds to a 5′-CAG-3′/5′-CAG-3′ triad, induced repeat contractions during DNA polymerase-mediated primer extension through the CAG repeat template. A thorough capillary electrophoresis and sequencing analysis revealed that the d(CAG)20 template gave shortened nascent strands mainly containing 3–6 CTG units in the presence of NA.