Simultaneous sensing of cysteine/homocysteine and glutathione with a fluorescent probe based on a single atom replacement strategy. Issue 11 (26th February 2021)

• Record Type: Journal Article
• Authors: Yao, Jingfeng; Yin, Guoxing; Yu, Ting; Li, Haitao; Yin, Peng
• Is Part Of: Analytical methods. Volume 13:Issue 11(2021)
• Page Start: 1358
• Page End: 1363
• Publication Date: 2021-02-26
• Subjects: Chemistry, Analytic -- Periodicals; Analytical biochemistry -- Periodicals; Chemical laboratories -- Standards -- Periodicals; 543.1905
• DOI: 10.1039/d0ay02206c
• Languages: English
• ISSNs: 1759-9660

Abstract

Abstract : Novel and simple fluorescent probes are utilized for the simultaneous monitor of Cys/Hcy and GSH. Abstract : In recent years, there have been many reports of fluorescent probes for multi-channel detection of Cys, Hcy and GSH. Particularly, reports of fluorescent probes using NBD (7-nitro-1, 2, 3-benzoxadiazole) or SNBD (7-nitro-1, 2, 3-benzothiadiazole) moieties as fluorophores are particularly common. Unfortunately, their 4-sulfhydryl derivatives exhibited negligible fluorescence, which makes them incapable of detecting GSH directly. Herein, by performing single selenium-for-oxygen atom replacement within 4-chloro-substituted NBD (NBD-Cl), we developed a small molecule fluorescent probe based on a single atom replacement strategy, which enables the probe to be used for simultaneously distinguishing Cys/Hcy and GSH, along with fluorescence imaging of Cys/Hcy and GSH in live cells from red and green emission channels, respectively.