Apoptosis oxidative damage‐mediated and antiproliferative effect of selenylated imidazo[1, 2‐a]pyridines on hepatocellular carcinoma HepG2 cells and in vivo. Issue 3 (30th October 2020)

• Record Type: Journal Article
• Authors: dos Santos, Daniela Coelho; Rafique, Jamal; Saba, Sumbal; Almeida, Gabriela M.; Siminski, Tâmila; Pádua, Cynthia; Filho, Danilo W.; Zamoner, Ariane; Braga, Antonio L.; Pedrosa, Rozangela C.; Ourique, Fabiana
• Is Part Of: Journal of biochemical and molecular toxicology. Volume 35:Issue 3(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-10-30
• Subjects: anticancer agent -- apoptosis -- DNA damage -- hepatocellular carcinoma -- oxidative stress -- selenium -- selenylated imidazo[1, 2‐a]pyridine; Biochemistry -- Periodicals; Molecular biology -- Periodicals; Toxicology -- Periodicals; 574
• DOI: 10.1002/jbt.22663
• Languages: English
• ISSNs: 1095-6670

Abstract

Abstract: Imidazo[1, 2‐ a ]pyridines (IP) and organoselenium compounds have been widely exploited in medicinal chemistry due to their pharmacological activities. Hepatocellular carcinoma (HCC) has few treatment options, and unfortunately, the prognosis is poor. Thus, the development of novel therapeutic drugs is urgent. The present study aimed at evaluating the antitumor mechanism of selenylated IP against HepG2 cells and in vivo. The selenylated IP named IP‐Se‐06 (3‐((2‐methoxyphenyl)selanyl)‐7‐methyl‐2‐phenylimidazol[1, 2‐ a ]pyridine) showed high cytotoxicity against HepG2 cells (half‐maximal inhibitory concentration [IC50 ] = 0.03 µM) and selectivity for this tumor cell line. At nontoxic concentration, IP‐Se‐06 decreased the protein levels of Bcl‐xL and increased the levels of p53, leading to inhibition of cell proliferation and apoptosis. This compound decreased the level of extracellular signal‐regulated kinase 1/2 protein and changed the levels of proteins involved in the drive of the cell cycle, tumor growth, and survival (cyclin B1, cyclin‐dependent kinase 2). In addition, IP‐Se‐06 decreased the number of cells in the S phase. In addition, IP‐Se‐06 led to increased generation of reactive oxygen species, changed antioxidant defenses, and caused DNA fragmentation. Finally, IP‐Se‐06 significantly inhibited the growth of Ehrlich ascites tumors in mice, increased survival time, and inhibited angiogenesis. Therefore, IP‐Se‐06 may be an important compound regarding the development of a therapeutic drug for HCC treatment. Abstract : The proposed mechanisms of antitumor action of selenylated imidazo[1, 2‐ a ]pyridine (IP‐Se‐06 ). The effects are the result of inhibition of extracellular signal‐regulated kinase (ERK) protein and oxidative attack on DNA that leads to the upregulation of p53. These combined effects induce inhibition of tumor growth and proliferation and cell death by apoptosis.