Engineered Methanotrophy: A Sustainable Solution for Methane-Based Industrial Biomanufacturing. Issue 4 (April 2021)

• Record Type: Journal Article
• Authors: Nguyen, Anh Duc; Lee, Eun Yeol
• Is Part Of: Trends in biotechnology. Volume 39:Issue 4(2021)
• Page Start: 381
• Page End: 396
• Publication Date: 2021-04
• Subjects: methanotrophic bacteria -- system-level understanding -- systems metabolic engineering -- synthetic methanotrophy -- methane; Biotechnology -- Periodicals; Biochemical engineering -- Periodicals; Genetic engineering -- Periodicals; Industrial microbiology -- Periodicals; 660.605
• DOI: 10.1016/j.tibtech.2020.07.007
• Languages: English
• ISSNs: 0167-7799

Abstract

Abstract : Methane is a promising feedstock with high abundance and low cost for the sustainable production of biochemicals and biofuels. Methanotrophic bacteria are particularly interesting platforms for methane bioconversion as they can utilize methane as a carbon substrate. Recently, breakthroughs in the understanding of methane metabolism in methanotrophs as well as critical advances in systems metabolic engineering of methanotrophic bacteria have been reported. Here, we discuss the important gaps in the understanding of methanotrophic metabolism that have been uncovered recently and the current trends in systems metabolic engineering in both methanotrophic bacteria and non-native hosts to advance the potential of methane-based biomanufacturing. Highlights: Engineering methanotrophy is a promising approach for sustainable production of chemicals and fuels from methane. Recently filled gaps in the understanding of methanotrophic metabolism are providing the groundwork for methane-based biomanufacturing. A systems metabolic engineering approach integrating systems biology, synthetic biology, and metabolic engineering has enabled the production of various products using methanotrophs. Synthetic methanotrophy is now being developed.