The proximity of the G-quadruplex to hemin impacts the intrinsic DNAzyme activity in mitochondria. Issue 24 (24th February 2021)

• Record Type: Journal Article
• Authors: Zhang, Jiayu; Song, Xiaofang; Xia, Miaoren; Xue, Yanan; Zhou, Mengxue; Ruan, Lifo; Lu, Huiru; Chen, Jun; Wang, Dongqi; Chai, Zhifang; Hu, Yi
• Is Part Of: Chemical communications. Volume 57:Issue 24(2021)
• Page Start: 3038
• Page End: 3041
• Publication Date: 2021-02-24
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/d0cc08316j
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : The DNAzyme activity in mitochondria is modulated by the proximity and interaction modes of the G-quadruplex with hemin. Abstract : The DNAzyme activity of G-quadruplex/hemin in mitochondria has not been characterized. Herein, we report an unexpected difference in the DNAzyme activity between in vitro assays and in mitochondria. Molecular dynamic simulations illustrate how the interaction of the G-quadruplex with hemin may modulate the DNAzyme activity. These results might facilitate a better understanding of the catalytic mechanism of the DNAzyme and help the rational design of stable and active DNAzymes suitable for intracellular catalysis.