Renoprotective effect of tectorigenin glycosides isolated from Iris spuria L. (Zeal) against hyperoxaluria and hyperglycemia in NRK-49Fcells. Issue 6 (19th March 2021)

• Record Type: Journal Article
• Authors: Divya, Ganesan; Albert, Abhishek; Singab, Abdel Nasser B.; Ayoub, Iriny M.; Al-Sayed, Eman; Paul, Eldho; Manoharan, Kumarriah; Saso, Luciano; Selvam, Govindan Sadasivam
• Is Part Of: Natural product research. Volume 35:Issue 6(2021)
• Page Start: 1029
• Page End: 1034
• Publication Date: 2021-03-19
• Subjects: Iris spuria L. -- tectorigenin derivatives -- antioxidant -- oxalate -- hyperglycemia -- hyperoxaluria; Natural products -- Periodicals; Natural products -- Research -- Periodicals; Biological Factors -- Periodicals; Biological Products -- Periodicals; 547
• DOI: 10.1080/14786419.2019.1613396
• Languages: English
• ISSNs: 1478-6419

Abstract

Abstract: Oxidative stress has been identified as an underlying factor in the development of insulin resistance, β-cell dysfunction, impaired glucose tolerance and type 2 diabetes mellitus and it also play major role in kidney stone formation. The present study is aimed to elucidate the in vitro nephroprotective activity of two isoflavonoid glycosides, tectorigenin 7- O- β-D-glucosyl-(1→6)-β-D-glucoside (1 ) and tectorigenin 7- O -β-D-glucosyl-4′- O -β-D-glucoside (2 ) isolated from the n -BuOH fraction of Iris spuria L. (Zeal) rhizome MeOH extract against oxalate and high glucose-induced oxidative stress in NRK-49F cells. The results revealed that compounds 1 and 2 significantly increased the antioxidant enzyme activities and decreased MDA levels in both oxalate and high glucose stress. Treatment with these phytochemicals effectively down-regulated expression of crystal modulator genes and pro-fibrotic genes in oxalate and high glucose-mediated stress respectively. This study indicates cytoprotective, antioxidant, anti-urolithic and anti-diabetic effects of compounds 1 and 2 against oxalate and high glucose stress.