Decellularized small intestine submucosa/polylactic-co-glycolic acid composite scaffold for potential application in hypopharyngeal and cervical esophageal tissue repair. Issue 2 (13th March 2021)

• Record Type: Journal Article
• Authors: Qiu, Shijie; Liang, Lijin; Zou, Peng; Chen, Qi
• Is Part Of: Regenerative biomaterials. Volume 8:Issue 2(2021)
• Publication Date: 2021-03-13
• Subjects: small intestine submucosa -- polylactic-co-glycolic acid -- hypopharyngeal and cervical esophageal cancer -- tissue repair -- composite scaffold; Biomedical materials -- Periodicals; Regenerative medicine -- Periodicals; 610.284
• DOI: 10.1093/rb/rbaa061
• Languages: English
• ISSNs: 2056-3418

Abstract

Abstract: There has been an increase in the incidence of hypopharyngeal and cervical esophageal cancer worldwide, and hence growing needs for hypopharyngeal and cervical esophageal tissue repair. This work produced a bi-layer composite scaffold with decellularized small intestine submucosa and polylactic-co-glycolic acid, which resembled the layered architectures of its intended tissues. The decellularized small intestine submucosa contained minimal residual DNA (52.5 ± 1.2 ng/mg) and the composite scaffold exhibited satisfactory mechanical properties (a tensile modulus of 21.1 ± 4.8 MPa, an ultimate tensile strength of 14.0 ± 2.9 MPa and a failure strain of 26.9 ± 5.1%). The interactions between cells and the respective layers of the scaffold were characterized by CCK-8 assays, immunostaining and Western blotting. Desirable cell proliferation and phenotypic behaviors were observed. These results have provided an important basis for the next-step in vivo studies of the scaffold, and bode well for its future clinical applications.