A Biomimetic, Copolymeric Membrane for Cell‐Stretch Experiments with Pulmonary Epithelial Cells at the Air‐Liquid Interface. (11th December 2020)

• Record Type: Journal Article
• Authors: Doryab, Ali; Taskin, Mehmet Berat; Stahlhut, Philipp; Schröppel, Andreas; Wagner, Darcy E.; Groll, Jürgen; Schmid, Otmar
• Is Part Of: Advanced functional materials. Volume 31:Number 10(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-12-11
• Subjects: alveolar‐capillary barrier -- cyclic mechanical stretch -- hybrid polymers -- in vitro cell‐stretch model -- tunable ultra‐thin biphasic membrane; Materials -- Periodicals; Chemical vapor deposition -- Periodicals; 620.11
• DOI: 10.1002/adfm.202004707
• Languages: English
• ISSNs: 1616-301X

Abstract

Abstract: Chronic respiratory diseases are among the leading causes of death worldwide, but only symptomatic therapies are available for terminal illness. This in part reflects a lack of biomimetic in vitro models that can imitate the complex environment and physiology of the lung. Here, a copolymeric membrane consisting of poly(ε‐)caprolactone and gelatin with tunable properties, resembling the main characteristics of the alveolar basement membrane is introduced. The thin bioinspired membrane (≤5 μm) is stretchable (up to 25% linear strain) with appropriate surface wettability and porosity for culturing lung epithelial cells under air–liquid interface conditions. The unique biphasic concept of this membrane provides optimum characteristics for initial cell growth (phase I) and then switch to biomimetic properties for cyclic cell‐stretch experiments (phase II). It is showed that physiologic cyclic mechanical stretch improves formation of F‐actin cytoskeleton filaments and tight junctions while non‐physiologic over‐stretch induces cell apoptosis, activates inflammatory response (IL‐8), and impairs epithelial barrier integrity. It is also demonstrated that cyclic physiologic stretch can enhance the cellular uptake of nanoparticles. Since this membrane offers considerable advantages over currently used membranes, it may lead the way to more biomimetic in vitro models of the lung for translation of in vitro response studies into clinical outcome. Abstract : A biphasic membrane concept is introduced, which changes its characteristic properties synchronized with the change in requirements for the initial cell growth phase versus subsequent cell‐stretch experiments. This simplifies cell culture procedures and enhances the biomimetic quality of lung‐on‐a‐chip and lung bioreactor studies.