A simple nuclear contrast staining method for microCT‐based 3D histology using lead(II) acetate. Issue 4 (2nd November 2020)

• Record Type: Journal Article
• Authors: Metscher, Brian
• Is Part Of: Journal of anatomy. Volume 238:Issue 4(2021)
• Page Start: 1036
• Page End: 1041
• Publication Date: 2020-11-02
• Subjects: 3D histology -- hematoxylin -- imaging -- lead(II) acetate -- limb development -- morphology -- staining -- X‐ray microtomography; Anatomy -- Periodicals; 571.3
• DOI: 10.1111/joa.13351
• Languages: English
• ISSNs: 0021-8782

Abstract

Abstract: X‐ray microtomography (microCT) enables histological‐scale 3D imaging of many types of biological samples, but it has yet to rival traditional histology for differentiation of tissue types and cell components. This report presents prima facie results indicating that a simple lead(II) acetate staining solution can impart preferential X‐ray contrast to cell nuclei. While not strictly selective for nuclei, the staining reflects local cell‐density differences. It can be applied in a single overnight treatment and does not require hematoxylin staining or drying of the sample. The stain is removable with EDTA, and it may enhance early calcifications. A basic protocol is given as a guide for further testing and optimization. Abstract : A simple lead acetate staining ("Bleikern") was found to impart useful X‐ray contrast to cell nuclei for microCT imaging. It can be applied in a single overnight treatment and allows histology‐like 3D imaging. The stain is removable with EDTA, and it may enhance early calcifications.