A novel electrochemical lung cancer biomarker cytokeratin 19 fragment antigen 21-1 immunosensor based on Si3N4/MoS2 incorporated MWCNTs and core–shell type magnetic nanoparticles. Issue 8 (23rd February 2021)

• Record Type: Journal Article
• Authors: Yola, Mehmet Lütfi; Atar, Necip; Özcan, Nermin
• Is Part Of: Nanoscale. Volume 13:Issue 8(2021)
• Page Start: 4660
• Page End: 4669
• Publication Date: 2021-02-23
• Subjects: Nanoscience -- Periodicals; Nanotechnology -- Periodicals; 620.505
• DOI: 10.1039/d1nr00244a
• Languages: English
• ISSNs: 2040-3364

Abstract

Abstract : Lung cancer is one of deadliest and most life threatening cancer types. Abstract : Lung cancer is one of deadliest and most life threatening cancer types. Cytokeratin 19 fragment antigen 21-1 (CYFRA 21-1) is a significant biomarker for the diagnosis of non-small cell lung cancer (NSCLC). Due to these reasons, a novel electrochemical immunosensor based on a silicon nitride (Si3 N4 )–molybdenum disulfide (MoS2 ) composite on multi-walled carbon nanotubes (Si3 N4 /MoS2 –MWCNTs) as an electrochemical sensor platform and core–shell type magnetic mesoporous silica nanoparticles@gold nanoparticles (MMSNs@AuNPs) as a signal amplifier was presented for CYFRA21-1 detection in this study. Capture antibody (Ab1 ) immobilization on a Si3 N4 /MoS2 –MWCNT modified glassy carbon electrode (Si3 N4 /MoS2 –MWCNTs/GCE) was firstly successfully performed by stable electrostatic/ionic interactions between the –NH2 groups of the capture antibody and the polar groups of Si3 N4 /MoS2 . Then, specific antibody–antigen interactions between the electrochemical sensor platform and the signal amplifier formed a novel voltammetric CYFRA21-1 immunosensor. The prepared composite materials and electrochemical sensor surfaces were characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). A linearity range of 0.01–1.0 pg mL −1 and a low detection limit (LOD) of 2.00 fg mL −1 were also obtained for analytical applications. Thus, the proposed immunosensor based on Si3 N4 /MoS2 –MWCNTs and MMSNs@AuNPs has great potential for medical diagnosis of lung cancer.