A characterization of heam uptake and intracellular distribution by isolated hepatocytes. Issue 7 (July 1985)

• Record Type: Journal Article
• Authors: Lodola, A.
• Is Part Of: Bioscience reports. Volume 5:Issue 7(1985)
• Page Start: 609
• Page End: 614
• Publication Date: 1985-07
• Subjects: Molecular biology -- Periodicals; Cytology -- Periodicals; 572.8
• DOI: 10.1007/BF01117074
• Languages: English
• ISSNs: 0144-8463

Abstract

Abstract : The uptake and intracellular distribution of haem by isolated rat hepatocyte suspensions was studied. An increase in cell haem content occurred after a challenge with 5, 10 or 20 μM haem, supplied as methaemalbumin. The rate of haem uptake was temperature dependent; no non-specific binding occurred. Intracellular haem distribution data are consistent with a rapid association of haem with the endoplasmic reticulum fraction prior to its accumulation in the cytosol and at the mitochondrion.