Novel signaling pathways regulate SARS-CoV and SARS-CoV-2 infectious disease. Issue 7 (19th February 2021)

• Record Type: Journal Article
• Authors: Cheng, Li-Chin; Kao, Tzu-Jen; Phan, Nam Nhut; Chiao, Chung-Chieh; Yen, Meng-Chi; Chen, Chien-Fu; Hung, Jui-Hsiang; Jiang, Jia-Zhen; Sun, Zhengda; Wang, Chih-Yang; Hsu, Hui-Ping
• Other Names: Wu. Bogang section editor.
• Is Part Of: Medicine. Volume 100:Issue 7(2021)
• Publication Date: 2021-02-19
• Subjects: bioinformatics -- coronavirus -- interleukin-17 -- severe acute respiratory syndrome coronavirus -- severe acute respiratory syndrome coronavirus-2 -- tumor necrosis factor; Medicine -- Periodicals; Medicine -- Periodicals; Médecine -- Périodiques; Geneeskunde; Medicine; Periodicals; Periodicals; 610.5
• DOI: 10.1097/MD.0000000000024321
• Languages: English
• ISSNs: 0025-7974

Abstract

Abstract : Abstract: Severe acute respiratory syndrome coronavirus (SARS-CoV)-2 induces severe infection, and it is responsible for a worldwide disease outbreak starting in late 2019. Currently, there are no effective medications against coronavirus. In the present study, we utilized a holistic bioinformatics approach to study gene signatures of SARS-CoV- and SARS-CoV-2-infected Calu-3 lung adenocarcinoma cells. Through the Gene Ontology platform, we determined that several cytokine genes were up-regulated after SARS-CoV-2 infection, including TNF, IL6, CSF2, IFNL1, IL-17C, CXCL10, and CXCL11 . Differentially regulated pathways were detected by the Kyoto Encyclopedia of Genes and Genomes, gene ontology, and Hallmark platform, including chemokines, cytokines, cytokine receptors, cytokine metabolism, inflammation, immune responses, and cellular responses to the virus. A Venn diagram was utilized to illustrate common overlapping genes from SARS-CoV- and SARS-CoV-2-infected datasets. An Ingenuity pathway analysis discovered an enrichment of tumor necrosis factor- (TNF-) and interleukin (IL)-17-related signaling in a gene set enrichment analysis. Downstream networks were predicted by the Database for Annotation, Visualization, and Integrated Discovery platform also revealed that TNF and TNF receptor 2 signaling elicited leukocyte recruitment, activation, and survival of host cells after coronavirus infection. Our discovery provides essential evidence for transcript regulation and downstream signaling of SARS-CoV and SARS-CoV-2 infection. Abstract : Supplemental Digital Content is available in the text