Impact of internal standard addition on dried blood spot analysis in bioanalytical method development. (October 2011)

• Record Type: Journal Article
• Authors: Meesters, Roland; Hooff, Gero; van Huizen, Nick; Gruters, Rob; Luider, Theo
• Is Part Of: Bioanalysis. Volume 3:Number 20(2011)
• Page Start: 2357
• Page End: 2364
• Publication Date: 2011-10
• Subjects: Drugs -- Analysis -- Periodicals; 615.19005
• DOI: 10.4155/bio.11.202
• Languages: English
• ISSNs: 1757-6180

Abstract

Background: Addition of internal standards to dried blood spot (DBS) specimens can be complicated. Therefore, we studied the feasibility of different internal standard addition procedures. Nevirapine and its stable-isotope analogue were used as model compounds and concentrations in DBS specimen were determined by matrix-assisted laser desorption/ionization-triple quadrupole tandem mass spectrometry using selected reaction monitoring.Results: The addition procedure of the stable isotope-labeled internal standard had significant impact on observed nevirapine concentrations. Relative recovery rates depending on the internal standard addition procedure ranged between 11.4 and 107.9%. Experiments with different punch sizes (5 and 7 mm diameter) showed no significant influence on observed nevirapine concentrations.Conclusion: Application of internal standard prior to blood spotting provided good nevirapine recoveries and this procedure is well suited for applying DBS in infectious diseases, especially in HIV-infection treatment.