Use of conventional bioanalytical devices to automate DBS extractions in liquid-handling dispensing tips. (October 2011)

• Record Type: Journal Article
• Authors: Johnson, Casey JL; Christianson, Chad D; Sheaff, Chrystal N; Laine, Derek F; Zimmer, Jennifer SD; Needham, Shane R
• Is Part Of: Bioanalysis. Volume 3:Number 20(2011)
• Page Start: 2303
• Page End: 2310
• Publication Date: 2011-10
• Subjects: Drugs -- Analysis -- Periodicals; 615.19005
• DOI: 10.4155/bio.11.212
• Languages: English
• ISSNs: 1757-6180

Abstract

Background: Conventional liquid-handling devices were employed, along with an improved punching device, to semi-automate dried blood spot (DBS) extraction of alprazolam, α-hydroxyalprazolam and midazolam from human whole blood. Liquid-handling devices were used to add internal standard to the DBS cards and to extract the analytes from the DBS, in order to be analyzed by HPLC–MS/MS.Results: The technique was shown to be accurate (±12.0%) and precise (10.3%) across the dynamic range of the assay.Conclusion: The semi-automated extraction reduced sample preparation time by more than 50% when compared with more conventional DBS manual extraction methods.