Analysis of total and transferrin-bound iron in human serum for pharmacokinetic studies of iron–sucrose formulations. (August 2011)

• Record Type: Journal Article
• Authors: Goggin, Melissa M; Gozum, Stephanie D; Burrows, David M; Nguyen, An; Corcoran, Henry F; Dotzauer, Heather; Tann, Cheng-Min; Lundberg, Richard A; Janis, Gregory C
• Is Part Of: Bioanalysis. Volume 3:Number 16(2011)
• Page Start: 1837
• Page End: 1846
• Publication Date: 2011-08
• Subjects: Drugs -- Analysis -- Periodicals; 615.19005
• DOI: 10.4155/bio.11.180
• Languages: English
• ISSNs: 1757-6180

Abstract

Background: Patients with iron-deficiency anemia benefit from intravenous iron therapies. Development of these pharmaceutical agents requires pharmacokinetic studies monitoring levels of both the administered agent and transferrin-bound iron (TBI). Successful pharmacokinetic methods must discriminate iron species.Results: Routine colorimetric procedures were used to reliably measure total iron and TBI following iron–sucrose administration. Iron was liberated from iron–sucrose allowing the determination of all circulating iron. Solid-phase sample processing allowed the measurement of TBI. Circulating iron–sucrose could then be calculated as the difference between total iron and TBI.Conclusion: A reproducible and robust spectrophotometric method was developed and validated for measuring total iron and TBI in human serum following iron–sucrose therapy.