A practical approach to reduce interference due to in-source collision-induced dissociation of acylglucuronides in LC––MS/MS. (August 2011)

• Record Type: Journal Article
• Authors: Mess, Jean-Nicholas; Béérubéé, Eugéénie-Raphaelle; Furtado, Milton; Garofolo, Fabio
• Is Part Of: Bioanalysis. Volume 3:Number 15(2011)
• Page Start: 1741
• Page End: 1751
• Publication Date: 2011-08
• Subjects: Drugs -- Analysis -- Periodicals; 615.19005
• DOI: 10.4155/bio.11.156
• Languages: English
• ISSNs: 1757-6180

Abstract

Background: In LC––MS/MS, glucuronide conjugated metabolites may convert back to the parent drug due to in-source collision-induced dissociation (CID).Results: During the bioanalysis of naproxen, it was noticed that naproxen acylglucuronide exhibited intense in-source CID to the naproxen [M+H] + ion under positive ESI. However, no in-source CID of the acylglucuronide to the naproxen [M+NH4 ] + adduct was observed. Furthermore, absolutely no in-source CID was detected under negative ESI. This phenomenon was not only observed for naproxen acylglucuronide but for eight other acylglucuronides compounds.Conclusion: We have shown that monitoring the parent drug [M-H] - or [M+NH4 ] + whenever possible could be an easy approach used by bioanalytical scientists to minimize the impact of in-source CID of acylglucuronides to the parent drug.