MicroRNA-196b promotes gastric cancer progression by targeting ECRG4. Issue 2 (February 2021)

• Record Type: Journal Article
• Authors: Chen, Liao; Tang, Huirong; Liu, Guobin; Xiao, Shufeng; Liang, Daoming; Ma, Jun; Yang, Yanlong; Luo, Haibo; Zhu, Yong; Xie, Fujia; Cheng, Xi; Chi, Junlin; Wu, Xuesong
• Is Part Of: Anti-cancer drugs. Volume 32:Issue 2(2021)
• Publication Date: 2021-02
• Subjects: gastric cancer -- ECRG4 -- invasion -- microRNA-196b -- migration -- proliferation; Antineoplastic agents -- Periodicals; Cancer -- Chemotherapy -- Periodicals; Antineoplastic Agents -- therapeutic use -- Periodicals; Drug Therapy -- Periodicals; 616.994061
• DOI: 10.1097/CAD.0000000000000998
• Languages: English
• ISSNs: 0959-4973

Abstract

Abstract : Gastric cancer is one of the most common malignant tumors. MicroRNA-196b (miR-196b) has been demonstrated to play important roles in human cancers. However, its functions in gastric cancer progression were still largely unknown. In this study, the expression of miR-196b was determined by quantitative real-time PCR. Esophageal cancer-related gene 4 ( ECRG4 ) level was examined by western blot assay and immunohistochemistry staining assay. Cell proliferation was evaluated by Cell Counting Kit-8 (CCK-8) assay and colony formation assay. Cell migration and invasion were analyzed by transwell assay. The association between miR-196b and ECRG4 was analyzed by dual-luciferase reporter assay. The functional role of miR-196b in vivo was analyzed by murine xenograft assay. As a result, we found the expression of miR-196b was elevated and the protein expression of ECRG4 was reduced in gastric cancer tissues and cells. MiR-196b inhibition suppressed gastric cancer cell proliferation, migration and invasion. ECRG4 was a target of miR-196b and its protein expression was negatively regulated by miR-196b. Moreover, ECRG4 overexpression showed similar effects with miR-196b inhibition on the malignant behaviors of GC cells and ECRG4 knockdown reversed the effects of miR-196b inhibition on gastric cancer cell proliferation, migration and invasion. In addition, miR-196b inhibition suppressed tumor volume and weight in vivo . In conclusion, downregulation of miR-196b inhibited gastric cancer progression by modulating ECRG4 expression, indicating that miR-196b might be a potential therapeutic target for gastric cancer.