Ionic dissolution products of Cerium-doped bioactive glass nanoparticles promote cellular osteogenic differentiation and extracellular matrix formation of human bone marrow derived mesenchymal stromal cells. (5th March 2021)

• Record Type: Journal Article
• Authors: Westhauser, F; Rehder, F; Decker, S; Kunisch, E; Moghaddam, A; Zheng, K; Boccaccini, A R
• Is Part Of: Biomedical materials. Volume 16:Number 3(2021)
• Publication Date: 2021-03-05
• Subjects: mesoporous bioactive glass nanoparticles -- cerium -- bone tissue engineering -- human mesenchymal stromal cells -- extracellular matrix; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1088/1748-605X/abcf5f
• Languages: English
• ISSNs: 1748-6041

Abstract

Abstract: Cerium (Ce) is a promising candidate ion for application in bone tissue engineering (BTE) since it reduces the presence of reactive oxygen species. Ce-doped mesoporous bioactive glass nanoparticles (MBGNs) serving as vectors for the local application of Ce already demonstrated stimulating effects on the expression of pro-osteogenic genes in Saos-2 cells. So far, there is no evidence available about the effects of Ce-doped MBGNs on the viability, osteogenic differentiation and the formation of the osseous extracellular matrix (ECM) of primary human bone marrow-derived mesenchymal stromal cells (BMSCs). Therefore, in this study, the biocompatibility of the ionic dissolution products (IDPs) of MBGNs containing increasing concentrations of CeO2 (0.05 MCe-MBGNs, composition in mol%: 86.6SiO2 -12.1CaO-1.3CeO2 ; and 0.2 MCe-MBGNs, composition in mol%: 86.0SiO2 -11.8CaO-2.2CeO2 ) and unmodified MBGNs (composition in mol%: 86SiO2 -14CaO) was evaluated using human BMSCs. Eventually, the impact of the MBGNs' IDPs on the cellular osteogenic differentiation and their ability to build and mature a primitive osseous ECM was assessed. The Ce-doped MBGNs had a positive influence on the viability and stimulated the cellular osteogenic differentiation of human BMSCs evaluated by analyzing the activity of alkaline phosphate as a marker enzyme for osteoblasts in the present setting. Furthermore, the formation and calcification of a primitive osseous ECM was significantly stimulated in the presence of Ce-doped MBGNs in a positive concentration-dependent manner as demonstrated by an elevated presence of collagen and increased ECM calcification. The results of this in-vitro study show that Ce-doped MBGNs are attractive candidates for further application in BTE.