Shapable bulk agarose–gelatine–hydroxyapatite–minocycline nanocomposite fabricated using a mineralizing system aided with electrophoresis for bone tissue regeneration. (3rd March 2021)

• Record Type: Journal Article
• Authors: Zhang, Heng; He, Xiaoxue; Zhang, Ya; Li, Quanli; Liu, Yueming; Zhang, Yiwen; Wang, Zonghua; Zhu, Qinghai; Li, Xiaofeng
• Is Part Of: Biomedical materials. Volume 16:Number 3(2021)
• Publication Date: 2021-03-03
• Subjects: hydrogel -- mineralization -- electrophoresis -- antimicrobial activity -- biocompatibility; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1088/1748-605X/abd050
• Languages: English
• ISSNs: 1748-6041

Abstract

Abstract: To develop a shapable bulk antibacterial nanocomposite biomaterial for bone regeneration. A bulk agarose–gelatine hydrogel was mineralized using a hydrogel mineralizing system aided with electrophoresis, and the mineralized hydrogel was loaded with minocycline to obtain the agarose–gelatine–hydroxyapatite–minocycline nanocomposite. The nanocomposite had a large Brunauer–Emmett–Teller surface area of 44.4518 m 2 g −1 and a high porosity of 76.9%. Hydroxyapatite crystals were well developed in the hydrogel matrix and exhibited a hybrid structure of microscale and nanoscale motifs. The addition of minocycline resulted in a continuous antibiotic release, inhibiting the growth of Staphylococcus aureus over 2 weeks in vitro . Exposed to rabbit bone marrow mesenchymal stem cells, the nanocomposite revealed good cytocompatibility in vitro . Furthermore, the biomaterial could effectively enhance the bone regeneration in a critical-size rabbit cranial defect model in vivo . These findings depicted that the nanocomposite, with good biocompatibility and good antibacterial property, is a promising candidate for future clinical application in bone tissue engineering or as a prospective bone replacement biomaterial.