Effect of magnesium ion concentration on two-dimensional structure of DNA-functionalized nanoparticles on supported lipid bilayer. (18th February 2016)

• Record Type: Journal Article
• Authors: Isogai, Takumi; Akada, Eri; Nakada, Sakiko; Yoshida, Naoya; Tero, Ryugo; Harada, Shunta; Ujihara, Toru; Tagawa, Miho
• Is Part Of: Japanese journal of applied physics. Volume 55:Number 3(2016:Mar.)Supplement 2
• Publication Date: 2016-02-18
• Subjects: Physics -- Periodicals; 621.05
• DOI: 10.7567/JJAP.55.03DF11
• Languages: English
• ISSNs: 0021-4922

Abstract

Abstract: The effect of divalent cations on lipid-bilayer-assisted DNA-functionalized nanoparticle (DNA-NP) assembly has been studied. We previously reported the lateral diffusion of DNA-NPs on planar lipid bilayer patches, owing to the mobility of lipid molecules in a supported lipid bilayer (SLB), and the resultant two-dimensional (2D) assembly of DNA-NPs. We here report the structural change of the assembled 2D DNA-NP lattices by magnesium ion concentration control on a successfully formed uniform SLB. In the magnesium-free buffer solution, DNA-NPs on SLB loosely assembled into quasi-hexagonal ordered lattices. In buffer solution containing 1 mM magnesium acetate, the interparticle distance of DNA-NPs decreased and the lattice structure became disordered. In buffer solution containing 5 mM magnesium acetate, the structure of DNA-NP arrays changed markedly and square lattices appeared. It is suggested that magnesium ions affected DNA molecules, which linked nanoparticles, and enabled the control of the structure of DNA-NP 2D arrays.