Pseudomonas paracarnis sp. nov., isolated from refrigerated beef. Issue 2 (22nd February 2021)

• Record Type: Journal Article
• Authors: Lick, Sonja; Wibberg, Daniel; Winkler, Annika; Blom, Jochen; Grimmler, Christina; Goesmann, Alexander; Kalinowski, Jörn; Kröckel, Lothar
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 71:Issue 2(2021)
• Publication Date: 2021-02-22
• Subjects: Pseudomonas -- meat -- beef -- dry ageing -- meat ripening; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.004652
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : During a project focusing on the diversity of meat microbiota associated with beef ripening, a Pseudomonas strain was isolated exhibiting high 16S rRNA gene sequence similarities (>99 %) to Pseudomonas carnis DSM 107652 T, P. lactis DSM 29167 T, P. paralactis DSM 29164 T and P. azotoformans DSM 18862 T . Phylogenetic analysis of the complete rpoB gene sequences of the isolate V5/DAB/2/5 T indicated a separate branch with about 99.0 % nucleotide identities to the closest relatives P. carnis DSM 107652 T, P. lactis DSM 29167 T and P. paralactis DSM 29164 T, while average nucleotide identities (ANIb) calculated from the draft genomes were 94.8, 94.2 and 90.2 %, respectively. Pairwise genome-to-genome distance calculations (GGDC) resulted in values of 67.7, 63.5 and 45.7 %, respectively, lying below the actual species demarcation line as well. A second isolate, UBT403, was detected some years later by using matrix-assisted laser desorption ionization-time of flight MS of the microbiota of minced beef. The fatty acid profile of V5/DAB/2/5 T consisted of C16 : 0, summed feature C 16 : 1 ω 7 c /iso-C15 : 0 2-OH, C18 : 1 ω7 c, C17 : 0 cyclo, C12 : 0, C12 : 0 3-OH, C10 : 0 3-OH and C12 : 0 2-OH. The major cellular lipids were aminopholipids, phospholipids, phosphatidylethanolamine and phosphatidylglycerol; the major quinone was Q9 with a minor proportion of Q8. Based on phenotypic and chemotaxonomic characterizations, the isolates can be considered as representing a novel species, for which the name Pseudomonas paracarnis sp. nov. is proposed. The type strain is V5/DAB/2/5 T (=DSM 111363 T =LMG 31846 T ); a second strain is UBT403 (=DSM 111362=LMG 31847).