Roseibium litorale sp. nov., isolated from a tidal flat sediment and proposal for the reclassification of Labrenzia polysiphoniae as Roseibium polysiphoniae comb. nov. Issue 2 (12th February 2021)

• Record Type: Journal Article
• Authors: Liu, Yang; Pei, Tao; Du, Juan; Chao, Meijie; Deng, Ming-Rong; Zhu, Honghui
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 71:Issue 2(2021)
• Publication Date: 2021-02-12
• Subjects: Roseibium litorale -- polyphasic taxonomy -- genotypes and phenotypes -- genomic analysis; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.004634
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A novel Gram-stain-negative, facultatively anaerobic, rod-shaped and non-motile bacterial strain, designated as 4C16A T, was isolated from a tidal flat sediment and characterized by using a polyphasic taxonomic approach. Strain 4C16A T was found to grow at 10–40 °C (optimum, 28 °C), at pH 5.0–10.0 (optimum, pH 6.0–7.0) and in 0–6 % (w/v) NaCl (optimum, 1 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 4C16A T fell into the genus Roseibium, and shared the highest identity of 98.9 % with the closest type strain Roseibium suaedae KACC 13772 T and less than 98.0 % identity with other type strains of recognized species within this genus. The phylogenomic analysis indicated that strain 4C16A T formed an independent branch within this genus. The 28.6 % digital DNA–DNA hybridization estimate and 85.0 % average nucleotide identity between strains 4C16A T and R. suaedae KACC 13772 T were the highest, but still far below their respective threshold for species definition, implying that strain 4C16A T should represent a novel genospecies. The predominant cellular fatty acid was summed feature 8; the polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylmonomethylethanolamine; the respiratory quinones were Q-9 and Q-10. The genomic DNA G+C content was 59.8mol %. Based on phylogenetic analyses and phenotypic and chemotaxonomic characteristics, strain 4C16A T is concluded to represent a novel species of the genus Roseibium, for which the name Roseibium litorale sp. nov. is proposed. The type strain of the species is 4C16A T (=GDMCC 1.1932 T =KACC 22078 T ). We also propose the reclassification of Labrenzia polysiphoniae as Roseibium polysiphoniae comb. nov. and ' Labrenzia callyspongiae ' as Roseibium callyspongiae sp. nov.