Aeromicrobium terrae sp. nov., isolated from a maize field. Issue 2 (5th February 2021)

• Record Type: Journal Article
• Authors: Lin, Shih-Yao; Tsai, Chia-Fang; Hameed, Asif; Young, Chiu-Chung
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 71:Issue 2(2021)
• Publication Date: 2021-02-05
• Subjects: Aeromicrobium terrae -- strain CC-CFT486T -- average nucleotide identity (ANI); Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.004616
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A polyphasic taxonomic approach was used to characterize a Gram-stain-positive bacterium, designated strain CC-CFT486 T, isolated from soil sampled in a maize field in Taiwan. Cells of strain CC-CFT486 T were short rods, motile with polar flagella, catalase-positive and oxidase-positive. Optimal growth occurred at 30 °С, pH 8 and 1 % NaCl. Phylogenetic analyses based on 16S rRNA genes revealed a distinct taxonomic position attained by strain CC-CFT486 T associated with Aeromicrobium panacisoli (97.0 % sequence identity), Aeromicrobium lacus (97.0 %), Aeromicrobium erythreum (96.8 %) and Aeromicrobium alkaliterrae (96.8 %), and lower sequence similarity values to other species. Average nucleotide identity (ANI) values were 70.6–77.8 % ( n =11) compared within the type strains of the genus Aeromicrobium . Strain CC-CFT486 T contained C16 : 0, C17 : 0, C17 : 1 ω 8c and C18 : 1 ω 9c as the predominant fatty acids. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, two unidentified aminophospholipids and three unknown phospholipids. The cell wall peptidoglycan of strains CC-CFT486 T contained ll -diaminopimelic acid (ll -DAP) and the major polyamine was spermidine. The DNA G+C content was 70.6 mol% and the predominant quinone was menaquinone 9 (MK-9). Based on its distinct phylogenetic, phenotypic and chemotaxonomic traits together with results of comparative 16S rRNA gene sequence and ANI analyses, strain CC-CFT486 T is proposed to represent a novel Aeromicrobium species, for which the name Aeromicrobium terrae sp. nov. (type strain CC-CFT486 T =BCRC 81217 T =JCM 33499 T ).