Characteristic synergistic cytotoxic effects toward cells in graphene oxide dressing with cadmium and copper ions. Issue 6 (9th October 2019)

• Record Type: Journal Article
• Authors: Dong, Yiyang; Chang, Yulin; Gao, Haidi; León Anchustegui, Victoria Arantza; Yu, Qiang; Wang, Haifang; Liu, Jia-Hui; Wang, Shihui
• Is Part Of: Toxicology research. Volume 8:Issue 6(2019)
• Page Start: 908
• Page End: 917
• Publication Date: 2019-10-09
• Subjects: Toxicology -- Periodicals; 615.9005
• DOI: 10.1039/c9tx00146h
• Languages: English
• ISSNs: 2045-452X

Abstract

Graphical Abstract: Abstract: The increasing applications of graphene oxide (GO) in bio-medicine, environment and other fields enhance the exposure possibility of human beings to GO. Studies have been performed to address the in vitro toxicity of GO; however, little information on the in vivo biological consequence of GO with other common disasters is available, especially when cells are co-exposed to GO and common metal ions. To explore the influence and possible mechanisms of such co-exposure scenarios, a series of tests of cell viability, membrane integrity, reactive oxygen species (ROS), cell morphology, and Cd 2+ distribution, were conducted. The results showed that the synergistic toxic mechanisms of GO and Cd 2+, initiated from the adhesion of GO on HeLa cells, and followed by the recruitment of Cd 2+ ions around the cell membrane, impaired the membrane integrity, morphology and adhesion capability, and triggered cell toxicity. The synergistic toxic mechanism of GO and Cu 2+ mainly correlated to ROS, while no obvious relationship with membrane integrity was observed. The findings are envisaged to facilitate the application of GO in biology and related fields.