Chondroitin sulphate at the endothelial lumen of pig aorta: Assay by radiolabelling and by X-ray microanalysis. Issue 3 (March 1987)

• Record Type: Journal Article
• Authors: Hale, G.; Streeter, H. B.; Hann, A. C.; Gacesa, P.; Wusteman, F. S.
• Is Part Of: Bioscience reports. Volume 7:Issue 3(1987)
• Page Start: 187
• Page End: 191
• Publication Date: 1987-03
• Subjects: chondroitin sulphate -- endothelium -- aorta -- X-ray microanalysis; Molecular biology -- Periodicals; Cytology -- Periodicals; 572.8
• DOI: 10.1007/BF01124788
• Languages: English
• ISSNs: 0144-8463

Abstract

Abstract : Trypsin-releasable glycosaminoglycans from the luminal surface of intact pig aorta were measured following metabolic labelling with 35 S]sulphate. Chondroitin sulphate was found to be present at a surface density equal to that already established for heparan sulphate (5×10 11 chains per cm 2 ). This result was confirmed by X-ray microanalysis of the luminal sulphur content before and after treatment with specific glycosaminoglycan-degrading enzymes. This result implies that approximately half of the luminal surface is occupied by sulphated glycosaminoglycans.