Systematic genetic analysis of transcription factors to map the fission yeast transcription-regulatory network. (20th November 2013)

• Record Type: Journal Article
• Authors: Chua, Gordon
• Is Part Of: Biochemical Society transactions. Volume 41:Number 6(2013)
• Page Start: 1696
• Page End: 1700
• Publication Date: 2013-11-20
• Subjects: fission yeast -- functional genomics -- Schizosaccharomyces pombe -- transcriptional-regulatory network -- transcription factor; Biochemistry -- Congresses; 572
• DOI: 10.1042/BST20130224
• Languages: English
• ISSNs: 0300-5127

Abstract

Abstract : Mapping transcriptional-regulatory networks requires the identification of target genes, binding specificities and signalling pathways of transcription factors. However, the characterization of each transcription factor sufficiently for deciphering such networks remains laborious. The recent availability of overexpression and deletion strains for almost all of the transcription factor genes in the fission yeast Schizosaccharomyces pombe provides a valuable resource to better investigate transcription factors using systematic genetics. In the present paper, I review and discuss the utility of these strain collections combined with transcriptome profiling and genome-wide chromatin immunoprecipitation to identify the target genes of transcription factors.