Membrane vesicles from brown adipose tissue: A tool for the study of amino acid transport. The case of L-alanine. Issue 2 (April 1992)

• Record Type: Journal Article
• Authors: Rodríguez-Martín, A.; Bel, N.; Remesar, X.
• Is Part Of: Bioscience reports. Volume 12:Issue 2(1992)
• Page Start: 115
• Page End: 122
• Publication Date: 1992-04
• Subjects: alanine -- brown adipose membrane vesicles -- transport; Molecular biology -- Periodicals; Cytology -- Periodicals; 572.8
• DOI: 10.1007/BF02351216
• Languages: English
• ISSNs: 0144-8463

Abstract

Abstract : A density gradient method is used to isolate membrane vesicles from brown adipose tissue. These respond to changes in osmolarity and show the classical overshoot pattern when L-alanine uptake is assayed. Transport is shown to be effected by two components: a linear (Kd =0.498 min −1 ) and Na + -dependent saturable component (Km =2.3 mM) and a Vmax =19.9 pmol/μg protein·min). This pattern is similar to that shown by cells isolated from brown adipose tissue.