A rapid and efficient method to enrich SAF-protein from scrapie brains of hamsters. Issue 2 (February 1984)

• Record Type: Journal Article
• Authors: Hilmert, Horst; Diringer, Heino
• Is Part Of: Bioscience reports. Volume 4:Issue 2(1984)
• Page Start: 165
• Page End: 170
• Publication Date: 1984-02
• Subjects: Molecular biology -- Periodicals; Cytology -- Periodicals; 572.8
• DOI: 10.1007/BF01120313
• Languages: English
• ISSNs: 0144-8463

Abstract

Abstract : Scrapie hamster brains contain at least 5–10 μg of scrapie-associated fibrils (SAF) per brain as estimated by the amount of its major constituent, a protein of about 26 000 daltons (SAF-protein). It can be extracted efficiently by a 10% solution of sarkosyl and can be enriched by differentia] centrifugation and buffer extraction. Scrapie infectivity, SAF, and SAF-protein copurify.