Agonist-induced calcium and oxidative stress responses in endothelial cells. (1st October 2003)

• Record Type: Journal Article
• Authors: Wilkinson, Jenny A.; Jacob, Ron
• Is Part Of: Biochemical Society transactions. Volume 31:Number 5(2003)
• Page Start: 960
• Page End: 962
• Publication Date: 2003-10-01
• Subjects: ATP -- calcium -- carboxy-2′, 7′-dichlorofluorescein -- endothelium -- mitochondria -- oxidative stress; Biochemistry -- Congresses; 572
• DOI: 10.1042/bst0310960
• Languages: English
• ISSNs: 0300-5127

Abstract

Abstract : [Ca 2+ ]i (cytosolic [Ca 2+ ]) and OS (oxidative stress) were measured simultaneously in calf pulmonary artery endothelial cells using fura-2 and carboxy-2´, 7´-dichlorodihydrofluorescein. ATP stimulated a [Ca 2+ ]i increase that was followed a few seconds later by an increase in OS. Pre-exposure to 5 μM H2 O2 potentiated these responses to ATP. Elevating or removing extracellular Ca 2+ increased or reduced the [Ca 2+ ]i response to ATP and caused parallel changes in the OS response, suggesting that this response was a consequence of the [Ca 2+ ]i response. Inhibition of mitochondria with rotenone or antimycin A affected the responses but not in a manner that allowed a simple interpretation of the role of mitochondria. These data show an initimate connection between [Ca 2+ ]i and OS that can be modulated by low levels of exogenously applied OS, allowing the possibility of positive feedback.