Functional dedifferentiation of parathyroid cells results both from a defective regulation and action of cytoplasmic Ca2+. Issue 12 (December 1986)

• Record Type: Journal Article
• Authors: Nygren, Peter
• Is Part Of: Bioscience reports. Volume 6:Issue 12(1986)
• Page Start: 1057
• Page End: 1063
• Publication Date: 1986-12
• Subjects: parathyroid hormone -- cytoplasmic Ca2+ -- culture -- hyperparathyroidism; Molecular biology -- Periodicals; Cytology -- Periodicals; 572.8
• DOI: 10.1007/BF01141027
• Languages: English
• ISSNs: 0144-8463

Abstract

Abstract : Monolayer culture of bovine parathyroid cells for 24 hours resulted in a right-shift of the dose-effect relationships for Ca 2+ -inhibition of parathyroid hormone (PTH) release and the dependence of the cytoplasmic Ca 2+ concentration (Ca 2+ ) on extracellular Ca 2+ as well as in a less suppressible hormone release. After 4 days of culture, hormone secretion was almost non-suppressible and Cai 2+ increased poorly in response to a rise in extracelluiar Ca 2+ . Ionomycin, a Ca 2+ ionophore, raised Cai 2+, but there was only a small inhibition of PTH release and the correlation between Cai 2+ and secretion was weak. A deteriorated Cai 2+ regulation and a decreased inhibitory action of cytoplasmic Ca 2+ on PTH release were also found in ceils from human parathyroid adenomas. Functional dedifferentiation of the parathyroid cell thus results from both defective regulation and action of cytoplasmic Ca 2+ .