Rapid response of protein synthesis to insulin in 3T3 cells: Effects of protein kinase C depletion and differences from the response to serum repletion. Issue 9 (September 1986)

• Record Type: Journal Article
• Authors: Hesketh, John E.; Campbell, Gillian P.; Reeds, Peter J.
• Is Part Of: Bioscience reports. Volume 6:Issue 9(1986)
• Page Start: 797
• Page End: 804
• Publication Date: 1986-09
• Subjects: insulin -- serum -- protein synthesis -- protein kinase C -- 3T3 fibroblasts -- signal transduction; Molecular biology -- Periodicals; Cytology -- Periodicals; 572.8
• DOI: 10.1007/BF01117102
• Languages: English
• ISSNs: 0144-8463

Abstract

Abstract : Quiescent 3T3 cells grown in media containing 4% foetal calf serum showed different responses to insulin and to serum repletion (to 12%). Insulin stimulated protein synthesis within 1 h and this early response was insensitive to actinomycin D. The later insulin response showed progressive sensitivity to actinomycin D. The serum response was slower, not occurring until 1 h, and was inhibited by actinomycin D. Depletion of cell protein kinase C by pre-treatment with phorbol ester caused a total block of the immediate response to insulin but had little effect on the response to serum or the later response to insulin. Acute phorbol ester treatment stimulated protein synthesis.