Vasoactive intestinal peptide (VIP) binding to solubilized material from rat liver plasma membranes. Issue 1 (January 1986)

• Record Type: Journal Article
• Authors: Guerrero, J. M.; Calvo, J. R.; Garrido, M. R.; Molinero, P.; Osuna, C.; Jiménez, J.; Goberna, R.
• Is Part Of: Bioscience reports. Volume 6:Issue 1(1986)
• Page Start: 39
• Page End: 44
• Publication Date: 1986-01
• Subjects: Molecular biology -- Periodicals; Cytology -- Periodicals; 572.8
• DOI: 10.1007/BF01145177
• Languages: English
• ISSNs: 0144-8463

Abstract

Abstract : A non-ionic detergent such as Lubrol-PX extracts in soluble form the VIP-binding structures of rat liver plasma membranes. Detergent-solubitized proteins bind specifically [ 125 I]VIP and the complex tracer-protein is identified by the use of Sepharose 6B columns. The interaction is only possible in the absence of detergent (below 0.001%) and is inhibited by native peptide. A molecular weight of about 80, 000 was estimated for VIP-binding proteins by reference to a series of globular markers of proteins. Binding to VIP soluble proteins is specific and dependent on time as studied by the Hummel and Dreyer ( Biochim. Biophys. Acta 63 :530–532, 1962) assay.