Recruitment of RNA polymerase to Class II CRP-dependent promoters is improved by a second upstream-bound CRP molecule. (25th October 2006)

• Record Type: Journal Article
• Authors: Miroslavova, N.S.; Mitchell, J.E.; Tebbutt, J.; Busby, S.J.W.
• Is Part Of: Biochemical Society transactions. Volume 34:Number 6(2006)
• Page Start: 1075
• Page End: 1078
• Publication Date: 2006-10-25
• Subjects: activation of transcription -- cAMP receptor protein (CRP) -- kinetic analysis -- recruitment -- RNA polymerase -- transcription; Biochemistry -- Congresses; 572
• DOI: 10.1042/BST0341075
• Languages: English
• ISSNs: 0300-5127

Abstract

Abstract : Genetics and biochemistry have been exploited to investigate transcription activation by the Escherichia coli CRP (cAMP receptor protein) factor at promoters with a DNA site for CRP near position −41 and the effects of a second upstream-bound CRP molecule. We show that the upstream-bound CRP contributes to transcription activation by improving the recruitment of RNA polymerase.